Protocol for nitrate/phophate estimation by HPLC
Published on by chitra pandey, National Institute Of Industrial Engineering, India - Research Scholar
What is an easy and efficient protocol for nitrate/phophate estimation by HPLC from soil and water samples?
2 Answers
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Please refer APHA book Standard methods for the examination of water and waste water. but Nitrate and phosphate are determined by spectrophotometric method.
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In my knowledge, there is no easy and efficient protocol for nitrate/phosphate estimation by HPLC. You have to make select HPLC Column by referring research papers. However, For Water Samples, you can use APHA Method 4110 (Ion-Cinematography). For Soil Samples, you can use Brucine-blue or yellow color methods for nitrates and Olsen’s Method for Phosphorous estimation.