Protocol for nitrate/phophate estimation by HPLC

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What is an easy and efficient protocol for nitrate/phophate estimation by HPLC from soil and water samples?

2 Answers

  1. Please refer APHA book Standard methods for the examination of water and waste water. but Nitrate and phosphate are determined by spectrophotometric method.

  2. In my knowledge, there is no easy and efficient protocol for nitrate/phosphate estimation by HPLC. You have to make select HPLC Column by referring research papers. However, For Water Samples, you can use APHA Method 4110 (Ion-Cinematography). For Soil Samples, you can use Brucine-blue or yellow color methods for nitrates and Olsen’s Method for Phosphorous estimation.