E. coli Test Method

I am a WASH ​manager in ​Pakistan and I ​would like to ​know how to ​properly ​conduct ​an  ​  Escherichia coli  test with the ​DelAgua kit (​Petri dish with ​an incubator) ​so we can check ​for  E. coli  in our water?

Please share ​the complete ​methodology for ​the  E. coli  test.

Taxonomy

• Bacteria
• E. coli
• Standards & Quality
• Water Quality
• Water Quality Management
• E. coli

9 Answers

1. Hello Ali,

   I invite you to look at our portable water quality testing kits that are used around the world. They are much easier for anyone to use than other tests on the market. Our kits are optimized for on-site testing in the field, especially in low resource, rural areas.

   The Aquagenx CBT Kit generates easy-to-score, quantified (MPN) test results for 100 mL samples WITHOUT needing laboratories, electricity, highly trained technicians or expensive, bulky equipment.

   We provide CBT E. coli Kits and CBT H2S Kits.

   We also provide portable field kits that include our E. coli tests as well as tests and equipment for key chemical and geophysical parameters.

   Please reach out to me for additional information!

   Lisa Hirsh, Aquagenx, lhirsh@aquagenx.com

2. You need 4-methylumbelliferyl-β-D-glucuronide  Escherichia coli  broth medium (Mug Agar), This can help you to achieve the desired result within 18 hrs, at 37 degrees celsius incubator.

   You will achieve the total coliform for which the blue dark E coli appear in the same place, where by you can easily do your count.

    

   use a 0.45 micron , filter paper.

    

3. Dear All .We have two email for emergency is gunawangumbiratno@gmail.com.  if you qusetion please send those email. sorry 

4. I have not used that test kit, either. One consideration when testing for E coli vs fecal coliforms (especially with some media) is the incubation temperature. Too high and you may inhibit E coli growth.

5. For on-site E.coli testing have a look at the Tecta-B16 automated, rapid detection system (US EPA approved for drinking water) at www.tecta-pds.com or contact me at patrick.wolfe@tecta-pds.com.  I've attached the datasheet here. 

6. Pb Agril University Ludhiana Punjab (India), HOD Microbiology Dr Sahota, mail ID hodmb@pau.edu have some simple patented techniques for all microbial tests. You may seek her help. May give my name as having given the reference. Best of luck. Deepinder

   1 Comment

   1. any details on that method, and why it is better than commercially available products? thanks

7. Try dip-testers, e.g. Millipore, that's usually the easyest and safest way under field conditions.

8. have you looked at 3M's Petrifilm or genx test (https://www.aquagenx.com/)? they have been recommended from field folks i work with.

9. I don't know that culture based product. It is much easier to use a Colilert kit.