The idea is a good? to identify the dominant nitrifying bacteria by NGS/QIIME2 procedure

Question

Dear all,
The ammonia nitrogen of wastewater is removed by nitrifying bacteria. We plan to adjust the part wastewater treatment equipment, the wastewater discharge replace to wastewater reuse, the  wastewater volume remain deal by the remain existing equipment, the loading will be higher, and that could affect the nitrification efficiency. 
We evaluate the cases by Lab pilot test, collect the sludge sample, and analysis the change of  dominant nitrifying bacteria by NGS/QIIME2 procedure, to decide the wastewater future to reuse or discharge. Is the idea good? and what is the risk?

Muthu Bharathi · Accepted Answer

I suggest you run the pilot & assess the effect of increased loading on the efficacy of nitration along with other parameters like TOC/COD vs NH3/NO2/NO3 ratios, change in pH, alkalinity, floc structure etc.
NGS etc can be done after evaluating the above crucial process parameters. & for troubleshooting issues in the Nitration efficiency of the pilot.

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