Untargeted metabolomics, also called discovery metabolomics, usually involves the comparison of the metabolome between the control and test groups, to identify differences between their metabolite profiles which may be relevant to specific biological conditions. There are usually three steps in the experimental workflow: 1 Profiling in order to seek the metabolites with statistically significant variations in abundance within a set of experimental and control samples; 2 Determination of metabolite ID, including the chemical structure; 3 Comprehensions as the last step which makes connections between the identified metabolites and the biological processes. In the workflow of discovery metabolomics, analytical reproducibility is critical for expression profiling work; annotation is a tentative identification based on an accurate mass match to a database or a spectral match to a library; the collected data can be interpreted for biomarker discovery, biological signature/fingerprint selection and pathway mapping. Above are the most important parts in untargeted metabolomic research. The tech panel in Creative Proteomics is experienced in sample preparation, data interpretations. With the advanced and well-maintained instruments, we can provide you reliable metabolite identification.